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Extra resources for Advances in Biochemical Engineering, Volume 9

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3. Pathway for Liquid Hydrocarbon Uptake . . . . . . . . . . . . . . 4. Hydrocarbon Pool . . . . . . . . . . . . . . . . . . . . . 5. Growth Kinetics of Microorganisms with Low Affinity for Liquid Hydrocarbon . . . 6. Growth Kinetics of Microorganisms with High Affinity for Liquid Hydrocarbon . . . 1 Transfer of Substrate During Clump Formation Accompanied Growth . . . . . 2 The Effect of Operating Conditions on the Growth Rate . . . .

Tropicalis that were desorbed is almost the same as that without Tween 20, and the fraction of cells of C. intermedia desorbed is lower than that with Tween 20 and higher than that without Tween 20. It is inferred from these results that C. intermedia secretes surface active agents but C. tropicalis does not. From these results it is assumed that the affinities of the hydrocarbon-utilizable microorganisms for hydrocarbon are different, depending on the kinds of microorganisms involved. Mechanismof Liquid HydrocarbonUptake by Microorganismsand Growth Kinetics 35 3.

However, the increase of lipid content on hydrocarbons did not reach the same level of accumulation as glycogen on glucose. In an N-free medium, both glycogen and lipids were accumulated. From these results the authors suggested that glycogen is not substituted by lipids as the carbon energy reserve on a hydrocarbon substrata. The relationships between the function and structure of the n-alkane-utllizing yeast cells were investigated by Hirai et al. [54], Osumi et al. [55, 56] and Teranishi et al.

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